|Title||Lung Innate Lymphoid Cell Composition is Altered in Primary Graft Dysfunction.|
|Publication Type||Journal Article|
|Year of Publication||2019|
|Authors||Monticelli LA, Diamond JM, Saenz SA, Wojno EDTait, Porteous MK, Cantu E, Artis D, Christie JD|
|Journal||Am J Respir Crit Care Med|
|Date Published||2019 Aug 08|
RATIONALE: Primary graft dysfunction (PGD) is the leading cause of early morbidity and mortality after lung transplantation but the immunologic mechanisms are poorly understood. Innate lymphoid cells (ILC) are a heterogeneous family of immune cells regulating pathologic inflammation and beneficial tissue repair. However, whether changes in donor-derived lung ILC populations are associated with PGD development has never been examined.
OBJECTIVES: To determine whether PGD in COPD or ILD transplant recipients is associated with alterations in ILC subset composition within the allograft.
METHODS: We performed a single center cohort study of lung transplantation patients with surgical biopsies of donor tissue taken prior to, and immediately following, allograft reperfusion. Donor immune cells from 18 patients were characterized phenotypically by flow cytometry for single-cell resolution of distinct ILC subsets. Changes in the percentage of ILC subsets with reperfusion or PGD (grade 3 within 72 hours) were assessed.
MEASUREMENTS AND MAIN RESULTS: Allograft reperfusion resulted in significantly decreased frequencies of natural killer (NK) cells and a trend toward reduced ILC populations, regardless of diagnosis (ILD or COPD). Seven patients developed PGD (38.9%), and PGD development was associated with selective reduction of the ILC2 subset after reperfusion. Conversely, non-PGD patients exhibited significantly higher ILC1 frequencies prior to reperfusion, accompanied by elevated ILC2 frequencies after allograft reperfusion.
CONCLUSIONS: The composition of donor ILC subsets is altered following allograft reperfusion and is associated with PGD development, suggesting that ILCs may be involved in regulating lung injury in lung transplant recipients.
|Alternate Journal||Am. J. Respir. Crit. Care Med.|